One of the strong features of this collection is the diversity of biosamples: frozen or paraffin-embedded tissue, biological fluids and derived products (nucleic acids and PBMC cells). In addition, the clinical data associated with the samples are enriched with a complete survey on personal and family history and exposures (smoking status and occupational exposure risks) as well as PET Scan data.
For each tissue sample, we store up to 5 healthy tissue fragments and 5 lesional tissue fragments at -80°C. For each tissue type, one of the fragments is dedicated to diagnostic use, and the others to research use. Additional tissue fragments may be included in paraffin (room temperature storage) and some may be included in the tissue blocks collection (storage at 4°C). The paraffin-embedded tissue blocks can, on request, enable the construction of collections dedicated to targeted pathologies as tissue microarrays (TMA).
Characterization by immunohistochemistry of paraffin-embedded tissue blocks can be performed on request, with a whole range of antibodies.
For the liquid biopsies of the thoracic collection, plasma, whole blood, serum and platelets are collected from patient blood samples and stored at -80°C.
From the blood samples, PBMC cells are also extracted and stored in liquid nitrogen. We also collect urine samples, stored at -80°C.
Derivatives are also prepared from the samples: constitutional DNA (extracted from whole blood), RNA, DNA from the tumor and plasma circulating tumor DNA.
Identification of genetic alterations from tissues, blood samples or directly from nucleic acids can be performed on request.